Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Sep 21;30(11):1663–1674. doi: 10.1038/s41594-023-01105-5

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2023, corrected publication 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Extended Data Fig. 1 — a – Diagram depicting domain map of Ub-CycB^N*. b, c – Cryo-EM data processing workflow showing representative micrographs from the APC/C-CDH1-UBE2C and APC/C-CDH1-UBE2C-UBE2S datasets (B). Particle detection was optimized using Topaz⁵⁹ and filtered using 3 rounds of 2D classification. Representative 2D projections from the final 2D classification step show the APC/C in multiple orientations (C). d – Local resolution map of the consensus refinements from the datasets. e – FSC curves showing resolution of the 3D density maps from the consensus refinement from the APC/C-CDH1-UBE2C and APC/C-CDH1-UBE2C-UBE2S datasets. Lines show the 0.143 gold-standard and 0.5 cut-off with values denoting resolution at 0.143 cut-off. f – Similar to Fig. 1d, fitted model of APC/C-CDH1-UBE2C. g – Overlay of APC/C-CDH1-UBE2C electron density map from this study with published maps from previous studies. Values shown indicate map-to-map correlation of maps from previous studies (EMD-2925 and EMD-2929) with APC/C-CDH1-UBE2C from this data after gaussian filtering^27,38. h – Overlay of electron density maps from the APC/C-CDH1-UBE2C and APC/C-CDH1-UBE2C-UBE2S consensus refinements, value shown indicates map-to-map correlation. i – Models showing UBE2S-CTP bound to the APC2/APC4 groove in previously described datasets (EMD-2925; EMD-3433)^23,27 and here in the APC/C-CDH1-UBE2C-UBE2S dataset.