Table 1.
Cryo-EM data collection, refinement and validation statistics
| #1 APC/C–CDH1–UBE2C | #2 APC/C–CDH1–UBE2C–UBE2S | |
|---|---|---|
| (EMD-41140), (PDB 8TAR) | (EMD-41142), (PDB 8TAU) | |
| Data collection and processing | ||
| Magnification | 180,000× | 180,000× |
| Voltage (kV) | 300 | 300 |
| Electron exposure (e− Å−2) | 42 | 42 |
| Defocus range (μm) | −0.7 to −3.5 | −0.7 to −3.5 |
| Pixel size (Å) | 0.82 | 0.82 |
| Symmetry imposed | C1 | C1 |
| Initial particle images (no.) | 2,001,820 | 1,894,448 |
| Final particle images (no.) | 661,289 | 774,933 |
| Map resolution (Å) | 4.0 Å | 3.5 Å |
| FSC threshold | 0.143 | 0.143 |
| Map resolution range (Å) | 3–10 Å | 3–10 Å |
| Refinement | ||
| Initial model used (PDB code) | 7QE7 | 7QE7 |
| Model resolution (Å) | 4.0 Å | 3.5 Å |
| FSC threshold | 0.143 | 0.143 |
| Model resolution range (Å) | 3–10 Å | 3–10 Å |
| Map sharpening B factor (Å2) | −188.3 | −159.6 |
| Model composition | ||
| Non-hydrogen atoms | 35,965 | 36,038 |
| Protein residues | 8,989 | 9,007 |
| R. m. s. deviations | ||
| Bond lengths (Å) | 0.006 | 0.007 |
| Bond angles (°) | 0.766 | 0.792 |
| Validation | ||
| MolProbity score | 1.70 | 1.81 |
| Clashscore | 6.84 | 9.17 |
| Ramachandran plot | ||
| Favored (%) | 98.19% | 97.89% |
| Allowed (%) | 1.81% | 2.12% |
| Disallowed (%) | 0.00% | 0.00% |