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. 2023 Sep 21;30(11):1640–1652. doi: 10.1038/s41594-023-01096-3

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 5 — a) Immunofluorescence of Hic1^{creERT2/creERT2}; Rosa26^SSM2/SSM2 mice at 16-week endpoint tongue tissue showing left, non-tamoxifen treated mice (-TAM) (upper panel) or tamoxifen treated mice expressing or not the SSM2 cassette (human SS18-SSX2) embedded in striated muscle +TAM; SSM2⁺ and +TAM; SSM2⁻ cells (lower panel). The cells are stained for DAPI, SSM2 and H2AK119ub1. The scale bar represents 100 um. b) Close-ups of images shown in the panel above, in the area delineated by the dashed square in (a). c) Quantification of H2AK119ub signal intensity normalised to DAPI signal intensity in 3 biological replicates (3 different mice) in non-tamoxifen treated mice (-TAM), or tamoxifen treated mice ( + TAM) expressing or not the SSM2 cassette (human SS18-SSX2) and showing normal tongue muscle ( + TAM; SSM2⁻) adjacent to synovial sarcoma tumours ( + TAM; SSM2⁺). Asterisks represent p-values of paired one-tailed t-test between groups (p = 0.0006). d) Spearman correlation between SS18-SSX, left or SSX, right signals and H2AK119ub1 signals per sarcoma sample.

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