Fig. 1.

Effect of BBR LCNPs in the reduction of WNT/β-catenin pathway genes β-catenin and Axin1. After 24-h treatment of A549 cells with BBR LCNPs, the expression of WNT/β-catenin pathway genes was assessed via RT-PCR, IF and immunoblotting. A β-Catenin expression, RT-PCR. B Axin1 expression, RT-PCR. The values are represented as mean ± SEM of 3 independent experiments, *p < 0.05, ***p < 0.001. C Immunofluorescence staining for β-catenin under 40 × magnification. D Fluorescence quantification with ImageJ Fiji. The values represent mean ± SEM of 3 independent experiments, ***p < 0.001. E β-Catenin expression, immunoblotting. F Pixel density quantification of immunoblotting with ImageJ Fiji. The values represent mean ± SEM of 3 independent experiments, ** p ≤ 0.01