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I-1-Pase expression and purification analyzed by SDS–12% PAGE stained with Coomassie brilliant blue. Lane a, crude extract; lane b, heat-treated crude extract; lane c, purified M. jannaschii I-1-Pase; lanes d and e, molecular mass standards (from the top, 66, 45, 36, 29, 24, 21.4, and 14 kDa).
