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(A) Thermal stability of M. jannaschii I-1-Pase after preincubation at 100°C for various times. The enzyme activity was measured after the preincubation time by adding 0.4 μg of protein to the standard assay mixture and incubating the mixture at 100°C for 1 min. (B) Temperature (T) dependence of M. jannaschii I-1-Pase Vmax. The error bars indicate standard deviations.
