TABLE 1.

Purification of M. jannaschii I-1-Pase

Step	Total protein (mg)	Sp acta	Total Ub	Yield (%)	Purification (fold)
Crude extract	870	2,300	2,000	100	1
Heat treatment	262	7,366	1,930	96.5	3.2
Q-Sepharose fast flow	128	8,632	1,150	57.5	3.75
Phenyl-Sepharose	91	9,876	900	45	4.3

^aIn nanomoles per minute per milligram. 

^bOne unit is defined as 1 μmol of P_i produced per mg of protein per min at 85°C.