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. 2023 Nov 13;136(12):244. doi: 10.1007/s00122-023-04492-x

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — Details of the genomic region including the most significant MTA with TKW (R2B.6). A Manhattan plot for chromosome 2B, including MTAs for Area (Ar), Perimeter (Perim), Major Ellipse (Majell), Minor Ellipse (Minell) and Thousand Kernel Weight (TKW; number of * indicate the number of years for which associations were found). P-values in this figure where corrected by Bonferroni (that is, multiplied by the number of independent tests performed). B Zoom of R2B.6. C Putative candidates and their expression values. * indicates expression > 0.5 TPM (For roots 1:radicle, 2: roots, 3: root apical meristem, 4: axillary roots; for leaves/shoots 1:coleoptile, 2: stem axis, 3: first leaf sheath, 4: first leaf blade, 5: shoot apical meristem, 6: third leaf blade, 7: third leaf sheath, 8: fifth leaf sheath, 9: fifth leaf blade, 10: shoot axis, 11: flag leaf blade, 12: leaf ligule, 13: flag leaf sheath, 14: Internode #2, 15: peduncle, 16: fifth leaf blade senescence, 17: flag leaf blade senescence; for spike 1: spike, 2: spikelets, 3: awns, 4: glumes, 5: lemma, 6: anther, 7: stigma & ovary; for grain 1: grain, 2: endosperm, 3: embryo proper, according to www.wheat-expression.com; Ramírez-González et al. 2018; Borrill et al. 2016)). D Average trait value according to the allele carried by the accessions in the most significant MTA. The picture illustrates the differences on grain size between ten landraces, five carrying allele A (BGE001942, BGE018217, BGE023723, BGE001983, BGE002012) and five allele G (BGE001945, BGE015402, BGE023725, BGE003236, BGE003156)