Table 2.
Primer names and sequences used in this study.
| Names* | Sequences | PCR conditions |
|---|---|---|
| PCSK9_ex1F | GAGGAGCTGGTGCTAGCCTTG |
94 °C 1 min 28 cycles of 94 °C 30 s 60 °C 30 s 72 °C 1 min 30 s |
| PCSK9_ex7R | GGCAAAGAGGTCCACACAGC | |
| PCSK9_ex6F | GACGATGCCTGCCTCTACTC | |
| PCSK9_ex12R | GTGCTGCCTGTAGTGCTGA | |
| SMN_F | AGGTCTCCTGGAAATAAATCAG |
55 °C 30 min 94 °C 2 min 25 cycles of 94 °C 30 s 56 °C 30 s 68 °C 1 min |
| SMN_R | TGGTGTCATTTAGTGCTGCTCT | |
| TBP_ex2F | AGCGCAAGGGTTTCTGGTTT |
55 °C 30 min 94 °C 2 min 24 cycles of 94 °C 30 s 58 °C 30 s 68 °C 1 min |
| TBP_ex3R | GGAGTCATGGGGGAGGGATA | |
| HNRNPD_ex1F | TTGACGCCAGTAAGAACGAG |
55 °C 30 min 94 °C 2 min 27 cycles of 94 °C 30 s 58 °C 30 s 68 °C 1 min |
| HNRNPD_in8R | ATACTGCTTCACCACCAAACG | |
| PCSK9 TaqMan expression assay | Hs.PT.58.20317141 |
95 °C 20 s 40 cycles of 95 °C 1 s 60 °C 20 s |
| TBP TaqMan expression assay | Hs00427620_m1 |
*PCSK9 transcript ID; NM_174936.4, SMN transcript ID; NM_017411.4, TBP transcript ID; NM_003194.5. HNRNPD transcript ID; NM_031370.3.