PCR cycling conditions
| Steps | Temperature | Time | Cycles |
|---|---|---|---|
| Initial Denaturation | 98°C | 45 s | 1 |
| Denaturation | 98°C | 15 s | Varied |
| Annealing | 65°C | 20 s | |
| Extension | 72°C | 60 s | |
| Hold | 4°C | hold | |
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