Ligation Mix-ddC

Reagent	Final concentration	Amount
10xRNA Ligase Buffer	0.94×	1.5 μL
50% v/v PEG8000	24%	7.5 μL
10 μM 3′-ddC adapter oligo	1.57 μM	2.5 μL
200 mM DTT	7.9 mM	0.625 μL
T4 RNA ligase 1 (10,000 U/mL)	2.36 U/μL	3.75 μL
Total	N/A	15.875 μL