cDNA Elution Mix

Reagent	Final concentration	Amount
10× RNase H Buffer	1×	5 μL
1 μM PE2 primer	20 nM	1 μL
50 mM D-biotin	12.5 mM	12.5 μL
RNase H (5,000 U/mL)	0.1 U/μL	1 μL
RNase cocktail enzyme mix	N/A	1 μL
Nuclease-free water	N/A	29.5 μL
Total	N/A	50 μL