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. 2023 Oct 24;14(5):2178–2191. doi: 10.21037/jgo-23-447

Figure 4.

Figure 4

HMGB2 facilitates the proliferative and migratory potential of hepatocellular carcinoma cells by activating the HMGB2-ZEB1-vimentin axis. (A) HMGB2 knockdown significantly downregulated ZEB1, vimentin, p53 and LEF1 protein expression levels in MHCC97H cells. (B) PCR was employed to evaluate ZEB1, vimentin, p53 and LEF1 mRNA expression levels following HMGB2 knockdown in MHCC97H cells. *, P<0.05 vs. control and scramble group. (C) Immunofluorescence staining with HMGB2, ZEB1 and vimentin antibodies, separately (scale bar =20 µm). DAPI staining solution (blue), Alexa Fluor 647 (red), Cy3 (orange), and Alexa Fluor 488 (green)-labeled secondary antibodies were used for detection. We repeated each experiment three times independently. Student t-test was employed for comparison between groups. HMGB2, high mobility group box 2; ZEB1, zinc finger E-box binding homeobox 1; LEF1, lymphoid enhancer-binding factor-1; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; siRNA, small interfering RNA; DAPI, 4’,6-diamidino-2-phenylindole; PCR, polymerase chain reaction.