TABLE 1.
Experiments carried out to investigate differences in environmental fitness between different SBW25 variants in soil and in the rhizospheres of pea and wheat
| Expt | SBW25 strain | Novel gene(s) | Insertion site | Environment |
|---|---|---|---|---|
| Comparison between wild type and triply marked recombinant | Wild type | None | Wheat phylloplane | |
| SBW25EeZY-6KX | lacZY | Ee | Wheat phylloplane | |
| SBW25EeZY-6KX | aph-1 xylE | -6- | Wheat phylloplane | |
| Effect of insertion site, tested in competition with SBW25EeZY-6KX | SBW25EeK | aph-1 | Ee | Rhizosphere of wheat |
| SBW25-6K | aph-1 | -6- | Rhizosphere of wheat | |
| Effect of metabolic load | SBW25-6K | aph-1 | -6- | Rhizospheres of wheat and pea |
| SBW25-6KX | aph-1 xylE | -6- | Rhizospheres of wheat and pea | |
| SBW25EeZY-6KX | aph-1 xylE | -6- | Rhizospheres of wheat and pea | |
| SBW25EeZY-6KX | lacZY | Ee | Rhizospheres of wheat and pea | |
| Effect of environment | SBW25-6K | aph-1 | -6- | Rhizospheres of wheat and pea, soil |
| SBW25-6KX | aph-1 xylE | -6- | Rhizospheres of wheat and pea, soil | |
| SBW25EeZY-6KX | aph-1 xylE | -6- | Rhizospheres of wheat and pea, soil | |
| SBW25EeZY-6KX | lacZY | Ee | Rhizospheres of wheat and pea, soil |