Fig. 2.

Procoagulant activity of human PBMCs in the presence of recombinant pneumolysin (a) or autolysin (b). PBMCs were isolated from human blood, and 1 × 10⁶ cells were incubated with pneumolysin or autolysin at indicated concentrations or 1 μg/mL LPS for 20 h at 37°C. Buffer-incubated cells were used as the control. Cell suspensions were then added to pre-warmed citrated human plasma and recalcified with 25 mM CaCl₂, and the time to form a clot was measured. Values represent the mean ± SD of four different donors, each done in duplicate. Asterisks indicate statistically significant differences as compared to the control. Significance was determined one-way ANOVA with Dunnett's posttest *p < 0.05; ***p < 0.001, ****p < 0.0001.