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. 2022 Jul 15;15(1):136–152. doi: 10.1159/000525479

Fig. 4.

Fig. 4

Adsorption of plasma proteins on the surface of pneumococci and mutant strains. Bacteria (1 × 1010 CFU/mL) were incubated with human plasma or PBS for 30 min at 37°C. After washing, bacteria-bound proteins were eluted with a glycine buffer (eluate). Activated plasma (plasma activated by DAPPTIN) and eluates were separated on SDS-PAGE, transferred to a membrane, and immunostained with polyclonal antibodies against HK, PK, FXII, fibrinogen alpha chain, or FVII. Experiments were done 3 times with pooled plasma, and one representative picture is shown.