Table 1.
Overview of cytokine and chemokine secretion (pg/mL unless indicated otherwise) or RALDH mRNA expression in the IEC-moDC-T-cell model when lECs were exposed to 2'FL and 3FL in absence of OVA
| IEC | Control | 2'FL | 3FL | IEC/DC | Control | 2'FL | 3FL | DC/T cel | Control | 2'FL | 3FL |
|---|---|---|---|---|---|---|---|---|---|---|---|
| IL33 | 1.4±1.2 | 7.0±3.7 | 2.2±1.3 | CCL22 (OD-value) | 0.3±0.03 | 0.2±0.03 | 0.2±0.09 | IL13 | 3.6±1.9 | 39.6±20.2 | 22.8±8.6 |
| TSLP | 8.4±2.9 | 11.5±2.5 | 9.5±4.0 | IL6 | 9.2±5.2 | 10.7±5.2 | 16.0±3.9 | IL9 | 4.1 ±2.1 | 10.0±7.9 | 8.7±4.7 |
| IL25 | 20.8±6.5 | 20.4±6.3 | 19.3±6.5 | IL8 | 86.3±45.4 | 45.0±22.4 | 31.1±12.0 | IL5 | 10.8±1.8 | 14.0±2.2 | 21.4±8.6 |
| CCL20 | 37.2±4.6 | 57.4±13.7 | 42.3±11.0 | IL15 | 261.9±22.5 | 207.2±42.24 | 132.9±38.81 | IFNγ | 3.3±1.0 | 30.01 ±24.2 | 98.4±88.3 |
| CCL22 | 159.8±28.7 | 191.3±34.9 | 180.6±29.9 | IL12p70 | 47.5±3.8 | 47.0±5.2 | 7.5±1.9** | IL25 (OD-value) | 0.33±0.05 | 0.37±0.1 | 0.69±0.14 |
| IL8 | 258.1 ±72.4 | 366.1 ±90.1 | 310.4±118.3 | IL23 | 112.4±18.8 | 73.8±9.6 | 59.9±41.5 | IL17 (OD-value) | 0.07±0.02 | 0.2±0.01 | 0.7±0.3 |
| Galectin-9 | 543.2±155.7 | 513.1±84.0 | 437.3±79.5 | Galectin-9 | 3,643±203.4 | 3,409±278.8 | 3,759±592.5 | Galectin-9 | 6,583±1,619 | 4,176±1,069* | 4,601 ±948.2* |
| TGFβ | 40.7±19.7 | 161.9±38.71 | 165.8±44.82 | TGFß | 27.5±6.3 | 120.3±38.23 | 32.3±13.0 | TGFβ | 263.7±27.0 | 614.1 ±28.2* | 673.8±79.2* |
| RALDH (Rel. Ab.) | 29.5±11.09 | 19.3±5.8 | 46.11±17.65 | IL10 | 11.3±1.8 | 17.0±1.7 | 12.4±0.6 | IL10 | 1.5±0.006 | 59.7±42.0 | 308.1 ±75.3* |
Data is analyzed by one-way ANOVA and Dunnet's post hoc tests were used to analyze statistical differences, n = 3, mean±SEM (* p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001).
1
p = 0.0943.
2
p = 0.0821.
3
p = 0.0756.
4
p = 0.0838.