Fig. 5.

IRF1 binding to the Mda5 promoter is required for Mda5 transcription. a Similar experiment to that in Fig. 4d, but the mutations in the canonical IRF-binding sequence were introduced in box 3. b–g In BMDM gene expression was determined by RT-PCR after LPS, IFN-α or poly(I:C) treatment for 3 h with the exception of f in which poly(I:C) treatment was not performed. E.C., electroporation control (n = 4). b Mda5, c Irf1, d Irf3, e Irf7, f Irf8, and g Irf9. Assays are representative of at least three independent experiments showing similar results. Each experiment was performed in triplicate, and the results are shown as the mean ± SD. **p < 0.01 and p < 0.0001 in relation to each stimulated sample compared to controls in each experiment. Data were analyzed using the unpaired Student's t test.