Table 1.
List of the primers used in qPCR
| Gene | NCBI reference | Forward primer (5′ to 3′) | Reverse primer (5′ to 3′) |
|---|---|---|---|
| Mda5 (Ifih1) | 71586 | GCCTGGAACGTAGACGACAT | TGGTTGGGCCACTTCCATTT |
| Ifn-β | 15977 | CAGCTCCAAGAAAGGACGA | GGCAGTGTAACTCTTCTGCA |
| Rig-I | 230073 | GGCTGAAAGCAAGGCTGATG | ACGCTATCAGATGTTGCCCC |
| Irf1 | 16362 | GAGATGTTAGCCCGGACACTTT | CCATATCCAAGTCCTGACCCA |
| Irf3 | 54131 | GAGAGCCGAACGAGGTTCAG | CTTCCAGGTTGACACGTCCG |
| Irf7 | 54123 | CCCCAGCCGGTGATCTTTC | CACAGTGACGGTCCTCGAAG |
| Irf8 | 15900 | TGACACCAACCAGTTCATCCGAGA | CACCAGAATGAGTTTGGAGCGCAA |
| Irf9 | 16391 | GCCGAGTGGTGGGTAAGAC | GCAAAGGCGCTGAACAAAGAG |
| c-myc | 17869 | AACAGCTTCGAAACTCTGGTGC | CGCATCAGTTCTGTCAGAAGGA |
| Arginase 1 | 11846 | TTGCGAGACGTAGACCCTGG | CAAAGCTCAGGTGAATCGGC |
| Tnf-α | 21926 | CCAGACCCTCACACTCAGATC | CACTTGGTGGTTTGCTACGAC |
| L14 | 68463 | TCCCAGGCTGTTAACGCGGT | GCGCTGGCTGAATGCTCTG |
| Hprt | 15452 | ATCATTATGCCGAGGATTTGG | GCAAAGAACTTATAGCCCCC |
| Sdha | 66945 | TGGGGAGTGCCGTGGTGTCA | CATGGCTGTGCCGTCCCCTG |
Primers were chosen based on their position regarding genomic sequence and, to avoid unspecific genomic DNA amplification, should be localized at a distance of 1 or more introns. Primers were designed using at the online Primer-Blast (NCBI).