Table 2.
Oligonucleotides used in the cloning of different plasmids
| Name | Sequence (5′ to 3′) |
|---|---|
| pCR2.1–1481 forward | AACGCTGTCACGAGGTTCAT |
| pCR2.1+384 reverse | TTTGTCCACCAAAGTGGGCT |
| pGL3–1481 forward | GGTACCGAGCTCAACGCTGTCACGAGGTTCAT |
| pGL3+384 reverse | TTACCGTTCGAATTTGTCCACCAAAGTGGGCT |
| pGL·box 1 upstream forward | CTATCGATAGGTACCGAGCTCAACGCTGTCACGAGGTTCA |
| pGL·box 1 upstream reverse | GAGGAACATTATCAGATAGTCACGTTTAGAAAAATTAATCTTTTAG |
| pGL·box 1 downstream forward | ACTATCTGATAATGTTCCTCCATAGCTGGTG |
| pGL·box 1 downstream reverse | CCAACAGTACCGGAATGCCATTTTGTCCACCAAAGTGGGC |
| pGL·box 2 upstream forward | CTATCGATAGGTACCGAGCTGGTACCGAGCTCAACGCTGTCAC |
| pGL·box 2 upstream reverse | TAATAGGGAGTCCAGACCTCCAGGGGGCGT |
| pGL·box 2 downstream forward | GAGGTCTGGACTCCCTATTAACTGACAAGTG |
| pGL·box 2 downstream reverse | GGTGGCTTTACCAACAGTAC |
| pGL·box 3 upstream forward | CTATCGATAGGTACCGAGCTGGTACCGAGCTCAACGCTGT |
| pGL·box 3 upstream reverse | TCGCTCTGCGAAGTTTCGTGAGGGTGGAGG |
| pGL·box 3 downstream forward | CACGAAACTTCGCAGAGCGAGAGCGGTCCC |
| pGL·box 3 downstream reverse | GGTGGCTTTACCAACAGTACCGGAATG |
| Mutated box 3 upstream forward | CCCCCCCCAGAACATTTCTCTATCG |
| Mutated box 3 upstream reverse | CAGATCGGCCCCTGCCCCGATTCTC |
| Mutated box 3 downstream forward | GAGAATCGGGGCAGGGGCCGATCTG |
| Mutated box 3 downstream reverse | GGGGGGGTGGCTTTACCAACAGTACCG |
| Fragment mutation upstream forward | CCCCCAGAACATTTCTCTATCG |
| Fragment mutation upstream reverse | ACAGTACCGGAATGCCAAGC |