Fig. 4.

Slc6a13 deficiency enhances the accumulation of glycine to decrease the secretion of inflammatory cytokines from macrophages infected by P. multocida. a, b The 20 most enriched cell types during P. multocida infection. The enrichment scores (labeled inside circle) is displayed in the −log10 of the Benjamini-Hochberg-adjusted p values. Cell types (labeled outside circle) with an enrichment score (red line) greater than 2 were considered significant. c, d The mRNA expression and supernatant protein concentration of IL-1β, TNF-α, IFN-γ, IL-6, and IL-12 in WT and KO macrophages infected or uninfected by PmCQ2 at 12 h (n = 3 or 6, one-way ANOVA). e The levels of glycine in WT and KO macrophages uninfected or infected by PmCQ2 at 12 h (n = 6, one-way ANOVA). f, g The mRNA expression and supernatant protein concentration of IL-1β, TNF-α, IFN-γ, IL-6, and IL-12 in WT and KO BAL macrophages with or without 10 mM glycine at 12 h postinfection (n = 6 or 8, one-way ANOVA). All data were expressed as means ± SD. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. SD, standard deviation.