Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 May 19;142(3):260–273. doi: 10.1182/blood.2022019451

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2023 by The American Society of Hematology. Licensed under Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0), permitting only noncommercial, nonderivative use with attribution. All other rights reserved.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

Figure 7. — Impact of ISB 1342 on circulating leukocytes and systemic soluble factors in cynomolgus monkeys. (A) Expression profile of CD38 on leukocyte populations from cynomolgus monkeys. Dots represent data the relative fluorescence intensity from each measurement and bars represent mean ± SD from 4 animals. Data were compared using a 1-way ANOVA followed by Dunnett post hoc comparison, ∗P ≤ .05. (B) Representative binding of ISB 1342 or isotype control on cynomolgus monkey B cells. (C-H) Cynomolgus monkeys (1 male and 1 female) were injected with 3 consecutive doses of ISB 1342 IV (1, 100 and 1000 μg/kg) at days 1, 29, and 57 respectively. Levels of peripheral B cells (C), monocytes (D), CD8⁺ T cells (E), activated CD8⁺ CD69⁺ T cells (F), CD4⁺ T cells (G), and activated CD4⁺ CD69⁺ T cells (H) were measured using flow cytometry. Data are mean ± SD of 10³ counts/μL normalized to baseline counts for 2 animals. (I-J) Levels of circulating IFN-γ (I) and ISB 1342 (J) were measured using ELISA. Data represent levels per animal and LLOQ is the lower limit of quantification for the assay. ELISA, enzyme-linked immunosorbent assay; LLOQ, lower limit of quantitation.

Figure 7. — Impact of ISB 1342 on circulating leukocytes and systemic soluble factors in cynomolgus monkeys. (A) Expression profile of CD38 on leukocyte populations from cynomolgus monkeys. Dots represent data the relative fluorescence intensity from each measurement and bars represent mean ± SD from 4 animals. Data were compared using a 1-way ANOVA followed by Dunnett post hoc comparison, ∗P ≤ .05. (B) Representative binding of ISB 1342 or isotype control on cynomolgus monkey B cells. (C-H) Cynomolgus monkeys (1 male and 1 female) were injected with 3 consecutive doses of ISB 1342 IV (1, 100 and 1000 μg/kg) at days 1, 29, and 57 respectively. Levels of peripheral B cells (C), monocytes (D), CD8⁺ T cells (E), activated CD8⁺ CD69⁺ T cells (F), CD4⁺ T cells (G), and activated CD4⁺ CD69⁺ T cells (H) were measured using flow cytometry. Data are mean ± SD of 10³ counts/μL normalized to baseline counts for 2 animals. (I-J) Levels of circulating IFN-γ (I) and ISB 1342 (J) were measured using ELISA. Data represent levels per animal and LLOQ is the lower limit of quantification for the assay. ELISA, enzyme-linked immunosorbent assay; LLOQ, lower limit of quantitation.