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. 1984 Dec;76(4):943–946. doi: 10.1104/pp.76.4.943

Depolarization of Cell Membrane Potential during Trans-Plasma Membrane Electron Transfer to Extracellular Electron Acceptors in Iron-Deficient Roots of Phaseolus vulgaris L. 1

Peter C Sijmons 1,2, Frank C Lanfermeijer 1,2, Albertus H de Boer 1,2, Hidde B A Prins 1,2, H Frits Bienfait 1,2
PMCID: PMC1064411  PMID: 16663976

Abstract

Transfer of electrons from the cytosol of bean (Phaseolus vulgaris L.) root cells to extracellular acceptors such as ferricyanide and FeIIIEDTA causes a rapid depolarization of the membrane potential. This effect is most pronounced (30-40 millivolts) with root cells of Fe-deficient plants, which have an increased capacity to reduce extracellular ferric salts. Ferrocyanide has no effect. In the state of ferricyanide reduction, H+ (1H+/2 electrons) and K+ ions are excreted. The reduction of extracellular ferric salts by roots of Fe-deficient bean plants is driven by cellular NADPH (Sijmons, van den Briel, Bienfait 1984 Plant Physiol 75: 219-221). From this and from the membrane potential depolarization, we conclude that trans-plasma membrane electron transfer from NADPH is the primary process in the reduction of extracellular ferric salts.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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