Reviewer name and names of any other individual's who aided in reviewer	Yasuhiro Go
Do you understand and agree to our policy of having open and named reviews, and having your review included with the published papers. (If no, please inform the editor that you cannot review this manuscript.)	Yes
Is the language of sufficient quality?	Yes
Please add additional comments on language quality to clarify if needed
Are all data available and do they match the descriptions in the paper?	Yes
Additional Comments
Are the data and metadata consistent with relevant minimum information or reporting standards? See GigaDB checklists for examples <a href="http://gigadb.org/site/guide" target="_blank">http://gigadb.org/site/guide</a>	Yes
Additional Comments
Is the data acquisition clear, complete and methodologically sound?	Yes
Additional Comments
Is there sufficient detail in the methods and data-processing steps to allow reproduction?	Yes
Additional Comments
Is there sufficient data validation and statistical analyses of data quality?	Yes
Additional Comments
Is the validation suitable for this type of data?	Yes
Additional Comments
Is there sufficient information for others to reuse this dataset or integrate it with other data?	Yes
Additional Comments
Any Additional Overall Comments to the Author	1. The value of repeat element content is 41.18% in the Abstract, but the Main Content value is 38.62%, which is inconsistent with the Abstract value. I would like to see the values be unified into one (Total value?). 2. Figure 1 should show not only a picture of the snake but also its distribution area (habitat). 3. The first sentence of the Result states "224.27 Gb long reads data," but single-tube long fragment read (stLFR) is not a true long read. The term "linked-read" is better. 4. Tables 3 and 4 do not have specific descriptions of "De novo," so please provide more details. 5. The authors use BUSCO to evaluate gene completeness, but I recommend trying compleasm (https://github.com/huangnengCSU/compleasm), a recently improved version of BUSCO. 6. The animals in the parentheses after "For the purpose of checking the quality of our assembly, six other kinds of amphibians and reptiles" in the "Data validation and quality control" section also use animals other than amphibians and reptiles, so please correct the sentence appropriately. 7. Figure 5C needs to be explained in the text. 8. There is no explanation of the meaning of the numbers in the branches of the phylogenetic tree in Figure 7. There needs to be an explanation of how they were obtained.
Recommendation	Minor Revision