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. 2023 Nov 14;18:74. doi: 10.1186/s13062-023-00433-2

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — MiR-5047 regulates OS stemness and malignancy by targeting SOX2. A. ENCORI, TargetScan and miRDB prediction of potential targets binding to miR-5047. Expression of indicated target genes was detected after transfecting the indicated miR-5047 mimics. B. Expression of SOX2 was detected in WAC-AS1-, miR-5047- or control-transfected cells. C. Hypothetical wild-type and mutant binding sites in miR-5047 for SOX2 3’UTR. Luciferase reporter assay detecting the binding of miR-5047 to SOX2 in OS cells. D. RIP with anti-AGO2 antibody was performed to detect WAC-AS1, miR-5047 and SOX2 mRNA. E. CCK8 assay showing the proliferation of OS cells following transfection of SOX2, miR-5047 + SOX2 or control. F. Colony formation of OS cells following transfection of SOX2, miR-5047 + SOX2 or control. G. Transwell assay showing the migration of miR-5047- or SOX2-transfected or control OS cells (scale bar, 50 μm). H. Wound healing assay the migration of SOX2 transfected, miR-5047-transfected or control OS cells (scale bar, 100 μm). I. Western blots showing stemness-related proteins in miR-5047-transfected, miR-5047 + SOX2-transfected or control cells. J. Changes in tumorsphere-forming ability of SOX2-transfected, miR-5047-transfected or control OS cells (scale bar, 50 μm). K. Percentage of CD133 + cells in populations of miR-5047-transfected, miR-5047 + SOX2-transfected or control OS cells was determined by flow cytometry. Error bars represent three independent experiments, *p < 0.05, **p < 0.01, *** p < 0.001