Figure 5.

Invasiveness of NEN cells in grafted zebrafish embryos. Overlay of representative fluorescent and bright field images of embryos grafted with red-stained BON-1 (A–C), NCI-H727 (D–F) and NCI-H720 (G–I) cells at 0 (A, D, G) and 48 hpi (B, C, E, F, H, I). For each injected cell line, the tail region was imaged at 48 hpi (C, F, I), showing the spread of NEN cells throughout the embryo body. All images are oriented so that rostral is to the left and dorsal is at the top. Scale bar, 100 μm. The graph J showed the quantification of NEN spread in the tail region at 48 hpi. NCI-H727 value has been set to 1.0. The graph K showed the quantification of NEN spread after 48 h of treatment with DMSO and CAB (0.25 and 2.5 µM). Control (DMSO) values have been set to 1.0. The values reported in the graphs represent the mean ± s.e.m. ^*P < 0.05; ^**P < 0.01; ^***P < 0.001. A full-colour version of this figure can be found at https://doi.org/10.1530/ERC-23-0232.