Key resources table
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Mouse anti-Bruchpilot antibody | Developmental Studies Hybridoma Ban | RRID:AB_2314866 |
| Rat, anti-CD8 monoclonal antibody | Thermo Fisher | RRID: AB_10392843 |
| Goat anti-mouse secondary antibody, Alexa Fluor 633 conjugate | Invitrogen | RRID:AB_141431 |
| Goat anti-rat secondary antibody, Alexa Fluor 488 conjugate | Fisher | RRID: A11006 |
| Mouse polyclonal anti-V5:DyLight 550 | AbD Serotec | Cat. No MCA1360D550GA |
| Rabbit polyclonal anti-HA | Cell Signaling Technologies | Cat. No 3724S |
| Rat monoclonal anti-FLAG | Novus Bio | RRID:AB_1625982 |
| Donkey anti-rat Alexa 647 | Jackson ImmunoResearch | RRID:AB_2340694 |
| Donkey anti-rabbit Alexa 594 | Jackson ImmunoResearch | RRID:AB_2340621 |
| Donkey anti-mouse Alexa 488 | Jackson ImmunoResearch | RRID:AB_2341099 |
| Rabbit-anti GFP polyclonal | Thermo Fisher | RRID: AB_2534023 |
| Goat anti-rabbit 488 | Thermo Fisher | RRID: AB_2633280 |
| Biological samples | ||
| Chemicals, peptides, and recombinant proteins | ||
| Texas Red™-X Phalloidin | Life Technologies | Cat. No T7471 |
| Calcofluor White | Sigma-Aldrich | Cat. No F3543-1G |
| Hoechst-33342 | Sigma-Aldrich | Cat. No 14533 |
| Critical commercial assays | ||
| Chromium Next GEM Single Cell 3’ v3.1 kit | 10x Genomics | Cat. No 1000269 |
| Deposited data | ||
| snRNA-seq of FeCO nuclei, raw data | This paper | GEO: GSE218661 |
| Experimental models: Cell lines | ||
| Experimental models: Drosophila strains | ||
| P{w[+mC]=iav-GAL4.K}3 | Bloomington | RRID:BDSC_52273 |
| P{JFRC7-20XUAS-IVS-mCD8::GFP} attp40 | Bloomington | RRID:BDSC_32194 |
| P{20xUAS-IVS-GCaMP6f} attP40; P{w[+mC]=UAS-tdTom.S}3 | Gift from Peter Weir and Michael Dickinson, Caltech | |
| P{GMR73D10-GAL4}attP2 | Bloomington | RRID:BDSC_39819 |
| P{GMR64C04-GAL4}attP2 | Bloomington | RRID:BDSC_39296 |
| P{GMR21D12-GAL4}attP2 | Bloomington | RRID:BDSC_48946 |
| P{UAS-RedStinger}3 | Bloomington | RRID:BDSC_8545 |
| P{lexAop-2xhrGFP.nls}2b | Bloomington | RRID:BDSC_29966 |
| Mi{Trojan-lexA:QFAD.0}ChAT[MI04508-TlexA:QFAD.0] | Bloomington | RRID:BDSC_60319 |
| P{y[+t7.7] w[+mC]= VT038873-p65ADZ}attP40 | Gift from Barry Dickson, Janelia Farm, HHMI | n/a |
| P{y[+t7.7] w[+mC]=R32H08-GAL4.DBD}attP2 | Bloomington | RRID:BDSC 69119 |
| P{y[+t7.7] w[+mC]= VT018774-p65ADZ}attP40 | Bloomington | RRID:BDSC 93430 |
| P{y[+t7.7] w[+mC]=VT040547-GAL4.DBD}attP2 | Gift from Barry Dickson, Janelia Farm, HHMI | n/a |
| P{y[+t7.7] w[+mC]=R92D04-p65.AD}attP40 | Bloomington | RRID:BDSC 70019 |
| P{y[+t7.7] w[+mC]=VT043140-GAL4.DBD}attP2 | Bloomington | RRID:BDSC 71960 |
| P{y[+t7.7] w[+mC]=R55C05-p65.AD}attP40 | Bloomington | RRID:BDSC 68650 |
| P{y[+t7.7] w[+mC]=VT017745-GAL4.DBD}attP2 | Bloomington | RRID:BDSC 73836 |
| P{y[+t7.7] w[+mC]= 50C12-p65ADZp}JK22C | Gift from Barry Dickson, Janelia Farm, HHMI | n/a |
| P{y[+t7.7] w[+mC]=R84H05-GAL4.DBD}attP2 | Bloomington | RRID:BDSC 69554 |
| P{y[+t7.7] w[+mC]=20XUAS-IVS-jGCaMP7f}VK00005 | Bloomington | RRID:BDSC 79031 |
| P{w[+mC]=UAS-tdTom.S}2 | Bloomington | RRID:BDSC 36327 |
| P{5xUAS-unc84-2xGFP}attp2 | Gift from Hongjie Li, Baylor | n/a |
| P{w[+mC]=nompA.GFP}5-3 | Bloomington | RRID:BDSC 42694 |
| Mi{PT-GFSTF.0}Piezo[MI04189-GFSTF.0] | Bloomington | RRID:BDSC 60209 |
| P{GMR IAV-LexA}attP2 | Gift from Gerry Rubin, Janelia Farm, HHMI | n/a |
| Mi{y[+mDint2]=MIC}Pzl[MI02586] | Bloomington | RRID:BDSC 60758 |
| P{pyx-GAL4.pro17} | Lei Liu, Qidong Fungene Biotechnology Co | n/a |
| P{w[+mC]=stum-GAL4.D}2 | Bloomington | RRID:BDSC 58776 |
| Mi{PT-GFSTF.0}Tmc[MI02041-GFSTF.0] | Bloomington | RRID:BDSC 60176 |
| tmem63-PGal4 | Gift from Craig Montell, UCSB | n/a |
| Recombinant DNA | ||
| Software and algorithms | ||
| Computational Morphometry Toolkit | Neuroimaging Informatics Tools and Resources Clearinghouse | https://www.nitrc.org/projects/cmtk/ |
| Matlab | MathWorks | RRID:SCR_001622 |
| ScanImage 5.2 | Vidrio Technologies | RRID:SCR_014307 |
| Fiji | Open-source | RRID: SCR_002285 |
| Cell Ranger Count (version 4.0.0) | 10x Genomics | |
| DecontX from Celda package (version 1.10.1) | https://bioconductor.org/packages/release/bioc/vignettes/celda/inst/doc/decontX.html | |
| DoubletFinder (version 2.0.3) | https://github.com/chris-mcginnis-ucsf/DoubletFinder | |
| Scanpy (version 1.9.1) | https://scanpy.readthedocs.io/en/stable/ | |
| COMSOL Multiphysics (version 6.1) | COMSOL | RRID:SCR_014767 |
| Other | ||