Figure 6.

Assessment of the role of the deep cervical tumor-draining lymph nodes in TIM-3 blockade

(A) Representative diagram of where the superior cervical (SC LN) and deep cervical lymph nodes (DC LN) are located.

(B) Flow cytometric analyses of the number of CD45⁺ immune cells in the DC LN on day 7 after i.t. treatment with IgG2a (black) or anti-TIM-3 (blue).

(C and D) Flow cytometric analyses of the numbers of (C) CD4⁺ T, B cells, (D) CD8⁺ T cells, and DCs in the DC LN on day 7 after i.t. treatment.

(E) MFI analysis of PD-L1 expression in DCs in the tumor-draining DC LN on Day 7 after treatment.

(F and G) Left panels, representative flow cytometry plots. Right panels, flow cytometric analysis of Ki67⁺, TNF-α⁺, GrzB⁺, and IFN-γ⁺ expression after ex vivo stimulation of (F) CD4⁺ or (G) CD8⁺ cells from the DC LN of mice treated with IgG2a or anti-TIM-3. Student’s t test was performed in all the graphs. Bar graphs indicate the mean ± SEM (ns, p > 0.05; ^∗p < 0.05; ^∗∗p < 0.01; ^∗∗∗p < 0.001; ^∗∗∗∗p < 0.0001). See also Figure S9.