Table 2.

Structures and structural predictions of alginate biosynthetic proteins.

Protein	UniProtKB	PDB code for structure	PDB code for structurally similar protein from DALI search	Function or predicted function	Fold or domains (residues)a	Reference
AlgD	P11759	1MFZ, 1MUU, 1MV8		GDP-mannose 6-dehydrogenase	Rossmann fold (1–202), connecting helix (203–234), (235–436)	Snook et al. (2003)
Alg8	Q52463		7SP8	Mannuronan synthase, GT	GT-A fold	Maloney et al. (2022)
Alg44	Q9HY69	4RT1, 4RT0	5C22	C-di-GMP-binding protein	PilZ domain (1–122), TM helix (152–185), membrane fusion protein (186–389)	Whitney et al. (2015), Kim et al. (2016)
AlgK	P96956	3E4B, 7ULA		Alginate export	TPR-containing protein	Keiski et al. (2010), Gheorghita et al. (2022a)
AlgE	P18895	3RBH, 4AZL, 4B61, 4AFK		Alginate export	β-barrel porin	Whitney et al. (2011), Tan et al. (2014)
AlgG	Q51371	4NK8, 4OZZ, 4OZY		C-5 mannuronan epimerase	Right-handed parallel β-helix fold	Wolfram et al. (2014)
AlgX	Q51372	4KNC, 7ULA		O-acetyltransferase	SGNH hydrolase-like domain (27–347), CBM domain (348–474)	Riley et al. (2013), Gheorghita et al. (2022a)
AlgL	Q06749	4OZV, 4OZW, 7SA8		Alginate lyase	(α/α)5 toroid fold	Gheorghita et al. (2022b)
AlgI	Q51392		6BUG	O-acetyltransferase	MBOAT	Ma et al. (2018)
AlgJ	Q51393	4O8V		O-acetyltransferase	TM helices (1–78), SGNH hydrolase-like	Baker et al. (2014)
AlgF	Q06062	6D10, 6CZT		Alginate acetylation, protein–protein interactions	β-sandwich fold (38–119, 124–212)	Low et al. (2023)
AlgA	P07874		2 × 65, 2PFW	PMI activity, GMP activity	Rossmann-like fold (1–272), left β-helix (279–318), cupin domain (358–463)	Pelissier et al. (2010)
AlgC	P26276	1K2Y, 1K35, 2FKF, 2FKM, 2H4L, 2H5A		Phosphomannomutase	Mixed α/β fold (1–153, 154–256, 257–368), TATA-box binding protein-like superfamily (369–463)	Regni et al. (2002, 2006a, b)

^aNumbering for P. aeruginosa protein.