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. 2023 Oct 31;14:1258023. doi: 10.3389/fpls.2023.1258023

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Copyright © 2023 Tselika, Belmezos, Kallemi, Andronis, Chiumenti, Navarro, Lavigne, Di Serio, Kalantidis and Katsarou

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Chop-PCR in non-infected and PSTVd-infected DCL3.10i N. benthamiana plants. (A) Northern blot analysis of DCL3.10i and PSTVd infected N. benthamiana plants (3wpi). Total RNA identified with methylene blue was used as a loading control. (*) Symbolizes the infected plants that were selected. Digestion of DNA from DCL3.10i non-infected and PSTVd infected plants with McrBC (B), HpaII (D), MspI (F), NlaIII (H), in triplicates of one biological repeat. Two more are present in Figure S4 . Used targets were a control DNA (chl), promoter region of cellulose synthase (cs), germin-like (gl), TE, ribosomal L18a (L18a), vironine synthase (vs), vacuolar protein sorting - associated protein (vtaI) and glucan endo 1-3 beta – glucosidase (1-3 beta). (C, E, G) and (I) Graphical representation of chop PCRs measurements. ‘n’ represents the number of analyzed samples from all biological and technical repeats. Results were analyzed with unpaired Student t-test, with level of significance set at p< 0.05 and symbolized with (*).