TABLE 1.
Isolation techniques and anaerobic growth capabilities of wild-type S. putrefaciens and Mnr mutants on Mn(IV) and alternate compounds as the sole terminal electron acceptor
| Strain | Isolation technique | Growtha
|
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| O2 | Mn(IV)b | Mn(IV) | Fumarate | NO2− | NO3− | TMAOc | Fe3+ | S2O32− | SO32− | ||
| 200 | + | + | + | + | + | + | + | + | + | + | |
| T121 | + | − | − | − | − | − | − | − | − | − | |
| 48-4 | 1 | + | − | − | + | + | + | + | + | + | + |
| 10-10 | 2 | + | − | − | + | + | + | + | + | + | + |
| 8-2 | 2 | + | − | ND | + | + | − | + | − | + | − |
| 10-22 | 2 | + | − | ND | + | + | − | + | − | − | − |
| 3-3 | 2 | + | − | ND | + | + | − | + | + | + | − |
| 9-19 | 1 | + | − | ND | + | + | − | + | + | + | − |
| 3-8 | 1 | + | − | ND | + | + | + | + | + | + | − |
| 19-10 | 1 | + | − | ND | + | + | − | + | − | − | + |
| 13-12 | 1 | + | − | ND | + | + | + | + | − | − | + |
a
−, unable to grow anaerobically on defined medium with the indicated compound as the sole terminal electron acceptor; +, able to grow anaerobically on defined medium with the indicated compound as the sole terminal electron acceptor; ND, not determined.
b
Mnr phenotype after application of screening techniques 1 and 2.
c
TMAO, trimethylamine N-oxide.