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. Author manuscript; available in PMC: 2023 Nov 14.
Published in final edited form as: Endocrinology. 2006 Jul 20;147(10):4753–4761. doi: 10.1210/en.2006-0196

Fig. 3.

Fig. 3.

Bone structure of the spinal column (A and B) and tibias (C and D) in IGF-I +/+ (A and C) and IGF-I −/− (B and D) embryos. Paraffin-embedded nondecalcified sections from 18.5-dpc embryos were stained by H&E staining. Only premature (prehypertrophic) chondrocytes locate in the spinal ossification center of the IGF-I −/− embryo (B). A similar morphological pattern and structure is seen in the long bones of the IGF-I +/+ and the IGF-I −/− embryos, but the size of the chondrocytes is smaller and the length of the hypertrophic zone is shorter in the IGF-I −/− embryo (D) than in the IGF-I +/+ embryo (C). Resting zone (RZ), proliferating zone (PZ), and hypertrophic zone (HZ) were distinguished by morphology of the cells in each zone. Bar, 50 μm.