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(A) Construction of mini-Tn5Kmtod-lux. A/X and Nh/X represent the AvrII-XbaI and NheI-XbaI heterologous cloning sites, respectively. N, NotI; Sa, SalI; X, XbaI. (B) Cloning plasmid pLJS with unique restriction sites. A, AvrII; Ac, AccI; Ap, ApaI; B, BamHI; Bs, BstXI; C, ClaI; D, DraII; E, EcoRI; Ea, EagI; EV, EcoRV; H, HindIII; Hc, HincII; K, KpnI; Nh, NheI; P, PstI; S, SpeI; Sc, SacI; ScII, SacII; Sm, SmaI; Xh, XhoI.
