Figure 1.

Temporal analysis of cell composition in two CLL patients. A. Cytotoxic CD8+ CAR T-cells expanded and dominated in the initial response phase mediating the killing of CLL cells, which remained undetected six months after CAR T-cell infusion. CD8+ CAR T-cells gradually declined and shifted to CD4+ CAR T-cells, which dominated at low levels over the second phase. In parallel, normal CD19+ B-cells gradually declined resulting in B-cell aplasia, while in the first patient B-cells gradually restored at 8 years post CAR T-cell infusion without disease recurrence. The actual CLL cell status remained unclear after six months, namely, whether the CLL burden was completely eradicated during the initial phase or some residual CLL cells remained over a decade and were destroyed by very few patrol-activated CD4+ CAR T-cells; B. temporal analysis of cell composition in two CLL patients. Estimation of the mean percentage of persisting CAR T-cells out of overall T-cells over two distinct phases in two CLL patients (based on Figure 1b, c [7] and Figure 2a [1], Figure 1b, c is a figure of [7], Figure 2a is a figure of [1], not this article). During the first six months post CAR T-cell infusion, the mean percentage of CAR T-cells out of all T-cells was approximately 35% in patient 1 and approximately 25% in patient 2. The corresponding percentages in the second phase were 0.217% in patient 1 and 1.266% in patient 2