Figure 3.

Fly grappa is broadly expressed in both larvae and adult flies

The gpp^TG4 allele was used to drive expression of UAS-fluorescent reporter transgene (UAS-mCherry.NLS).

(A) gpp is expressed broadly and highly throughout the body in both larvae and adult flies. Nuclear mCherry was present and whole larvae or adult was imaged.

(B) Schematic of different tissues of larvae.

(C) gpp is expressed in most tissues in larvae. Nuclear mCherry was present and tissue were dissected from L3 larvae including eye, leg, wing, and haltere discs.

(D and E) gpp is broadly expressed in the nervous system. Nuclear mCherry was driven by gpp^TG4/+ and tissues were dissected from L3 larvae (CNS, includes central brain and VNC) or adults (brain). Shown is half of the adult brain. Tissue was counterstained with markers for neurons (Elav) (D) or glia (Repo) (E). Z stacked images of gpp expression pattern compared to neurons or glia are shown in the left panels in (D) and (E), respectively. Single slice images (right panels) were used to better visualize cellular co-localization of Nuclear mCherry signal with neurons (D) or glia (E). Note that gpp is expressed in a large subset of neurons and in a small subset of glia. VNC, ventral nerve cord; OL, optic lobe.