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. 2023 Oct 31;11:1270398. doi: 10.3389/fcell.2023.1270398

FIGURE 2.

FIGURE 2

(A) Stromal cells were cultured on the bottom compartment of a transwell plate. Tumour cells were added to the upper compartment for 1 h and treated with Obz for 23 h. Survival percentages in direct contact conditions [shown in (B) for Raji, (C) for Daudi] were calculated by labelling cells with 7-AAD and AnnexinV. Survival percentages for a control assay, were cells were plated in non-contact conditions with the stromal layer, are shown in (D) for Raji and (E) for Daudi. (F) Tumour cells were cultured either on plastic or on a layer of PKH67-labelled M2-10B4 cells for 24 h. Cells were separated through the use of a FACS sorter and PKH67- tumour cells were plated on plastic. Cells were treated with Obz on plastic for further 4 h either immediately after sort (0 h post-sort) or 4, 16 or 20 h post-sort. Survival percentages, shown in (G), were calculated by labelling cells with 7-AAD and AnnexinV. Data is mean ± SEM of two to four independent experiments.