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. 2023 Oct 31;11:1270398. doi: 10.3389/fcell.2023.1270398

FIGURE 3.

FIGURE 3

(A) Raji cells were cultured either on plastic (left panel) or on a layer of M2-10B4 stromal cells (middle panel) and treated with Obinutuzumab (Obz) at 10 μg/mL. Images were taken on a Zeiss lowlight microscope (10X) 24 h later. Non-treated Raji cells cultured on plastic are shown in the right panel. (B) Raji cells were pre-treated with Obz in tubes for 2 h and poured onto either plastic (left panels) or onto a layer of M2-10B4 stromal cells (right panels). Images were taken on a Zeiss lowlight microscope (10X) either straight after the pouring (upper panels) or 24 h later (lower panels). (C) Raji cells were cultured either on plastic (black bars) or onto a layer of M2-10B4 stromal cells (grey bars) and either left untreated (NT) or treated with Obz at 10 μg/mL (Obz) or pre-treated with Obz at 10 μg/mL for 2 h (Obz 2h) in a tube and then poured onto the wells. Survival percentages were calculated by labelling cells with 7-AAD and AnnexinV. Data is mean ± SEM of at least 3 independent experiments.