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. 2023 Oct 31;11:1270398. doi: 10.3389/fcell.2023.1270398

FIGURE 4.

FIGURE 4

(A,B) Raji-GFP-actin cells (green) were cultured either on plastic (upper panels) or on a layer of mCherry-M2-10B4 stromal cells (orange, lower panels) and either left untreated (A) or treated with Obz at 10 μg/mL (B) for 24 h. Nuclei (blue) were labelled with Hoechst 33,342 (0.1 μg/mL) and plates were scanned on a PerkinElmer Opera Phenix (20X) 24 h later. (C) The signal intensity to area ratio for Raji-GFP-actin cells was calculated using Columbus software. (D–E) Raji-YFP-CD20 cells (green) were cultured either on plastic (upper panels) or on a layer of mCherry-M2-10B4 stromal cells (orange, lower panels) and either left untreated (D) or treated with Obz at 10 μg/mL (E) for 24 h. Nuclei (blue) were labelled with Hoechst 33,342 (0.1 μg/mL) and plates were scanned on a PerkinElmer Opera Phenix (20X) 24 h later. (F) The signal intensity to area ratio for Raji-YFP-CD20 cells was calculated using Columbus software. **p < 001 unpaired t-test. Data is mean ± SEM of at least 3 independent experiments.