FIGURE 7.

Raji cells were grown in RPMI media containing either heavy (Raji-H) or medium (Raji-M) isotopes of Lysine and Arginine. Cells were cultured either on plastic (Raji-M) or on a layer of PKH67-labelled M2-10B4 stromal cells (Raji-H) for 24 h. Tumour cells were separated from stromal cells by FACS sorting and the resulting populations of Raji-M and Raji-H were lysed. Protein content was calculated and lysates from Raji-M and Raji-H were mixed in a 1 to 1 ratio before being resolved in a SDS-PAGE and analysed by mass spectrometry. (A) Protein IDs which had p-value <0.05 and had expression fold change < -2 or >2 were uploaded in DAVID. The cluster annotation tool was used to identify the mostly enriched clusters of pathways which were differentially expressed in Raji-H. (B) Protein IDs which had p-value <0.05 and expression fold change < -2 or >2 were uploaded in IPA. Pathways which were altered in Raji-H compared to Raji-M are shown. For each of them, their significance, ratio and Z-score are shown. Data is from one experiment.