TABLE 1.
Strains used in this study.
| Strains | Relevant characteristics | References |
|---|---|---|
| Pseudomonas putida | ||
| KT2440 | Wild-type strain derived from P. putida mt-2 cured of the pWW0 plasmid | Bagdasarian et al. (1981) |
| PP05_01 | KT2440 derivative strain with pha cluster deleted (PP_5003-PP_5008) _CECT 30020 | Mato et al. (2020) |
| PP00_02 | Kmr, PP05_01 derivative strain expressing Ptrc:phaC Cn -phaA Cn -phaB Cn genes, integrated into the genome (via mini-Tn5 transposon) using the pMAB26 plasmid | This work |
| PP00_03 | Kmr, KT2440 strain expressing Ptrc:phaC Cn -phaA Cn -phaB Cn genes integrated into the genome (via mini-Tn5 transposon) using the pMAB26 plasmid | This work |
| PP01_02 | Gmr, PP05_01 derivative strain harboring 14f:BCD2-phaC Cn -rnpBT1; 14a:BCD2-phaA Cn -rpoC;14a:BCD2-phaB Cn -λT1 at attTn7 site | This work |
| PP05_12 | Gmr, PP05_01 derivative strain harboring 14a:BCD2-phaC Cn -rnpBT1; 14a:BCD2-phaA Cn -rpoC;14a:BCD2-phaB Cn -λT1, at attTn7 site | This work |
| PP05_15 | Gmr, PP01_02 derivative strain with ibpA locus deleted (PP_1982) | This work |
| PP05_16 | Gmr, PP05_01 derivative strain harboring 14a:BCD2-phaC Cn -rnpBT1; 14a:BCD2-phaA Cn -rpoC;14a:BCD2-phaB Cn -λT1; 14a:BCD2-phaP1 Cn -rnpBT1, at attTn7 site | This work |
| KT2440 (pGG128) | Kmr, KT2440 strain harboring pGG128 empty plasmid | This work |
| PP05_01 (pGG128) | Kmr, PP05_01 strain harboring pGG128 empty plasmid | This work |
| PP05_01 (pSS126) | Kmr, PP05_01 strain harboring pSS126; 14a:BCD2-phaC2 Rr -λT0; 14a:BCD2-phaA Rr -λT0; 14a:BCD2-phaB Rr - λT0 | This work |
| PP05_01 (pRK216) | Kmr, PP05_01 strain harboring pRK216; SynPro16-phaC Cn -rnpBT1; SynPro16-phaA Cn -rpoC; SynPro16-phaB1 Cn -T500 | This work |
| PP05_01 (pMM85) | Kmr, PP05_01 strain harboring pMM85; 14a:BCD2-phaC Cn -rnpBT1; 14a:BCD2-phaA Cn -rpoC; 14a:BCD2-phaB Cn -λT1; 14a:BCD2-phaP1 Cn -rnpBT1 | This work |
| PP05_01 (pMM106) | Kmr, PP05_01 strain harboring pMM106; 14a:BCD2-phaC5 Pp -rnpBT1; 14a:BCD2-phaA3 Pp -rpoC; 14a:BCD2-phaB Pp -λT1; 14a:BCD2-phaP1 Pp -rnpBT1 | This work |
| PP05_12 (pMM194) | Kmr, Gmr, PP05_12 strain harboring pMM194; 14a:BCD2-ibpA Pp -rnpBT1 | This work |
| PP05_15 (pMM194) | Kmr, Gmr, PP05_15 strain harboring pMM194; 14a:BCD2-ibpA Pp -rnpBT1 | This work |
| PP01_02 (pBDN2-GFP) | Kmr, PP01_02 strain harboring pBDN2-GFP, empty plasmid | Blanco et al. (2023) |
| PP01_02 (pBDN2-PhaP) | Kmr, PP01_02 strain harboring pBDN2-PhaP, harboring the PhaP1 Cn | Blanco et al. (2023) |
| Escherichia coli | ||
| DH5αλpir | Tcr, cloning host; DH5α lysogenized with λpir phage. Host strain for oriR6K plasmids | Zobel et al. (2015) |
| DH10B | Spr, cloning host; F-, mcrA Δ(mrr hsdRMS-mcrBC) Φ80dlacΔM15 ΔlacX74 deoR recA1 araD139 Δ(ara-leu)7697 | Invitrogen, Thermo Fisher Scientific, United States of America |
| HB101 (pRK600) | Cmr, Conjugation helper strain; F− λ− hsdS20(r B - m B - ) recA13 leuB6(Am) araC14 Δ(gpt-proA)62 lacY1 galK2(Oc) xyl-5 mtl-1 rpsL20(Smr) glnX44 (AS) | Boyer and Roulland-Dussoix (1969) |
| R. rubrum ATCC 11170 | R. rubrum type strain | Pfennig and Trüper (1971) |
| C. necator H16 DSM 428 | C. necator H16 | Makkar and Casida (1987) |