TABLE 3.

Properties of heterologous PHA production in Pseudomonas putida chassis strain. GC-MS analysis of PHA content in 0.1 N M63 minimal medium supplemented with 15 mM octanoate for 24 h. The data correspond to the mean values and standard deviations of at least two independent biological replicates. TR.: Traces; N.D.: not detected.

Strain	Total CDW (g/L)	PHA (%CDW)	PHA (g/L)	Residual biomass (g/L)	%C4	%C6	%C8
KT2440 (pGG128; empty plasmid)	1.42 ± 0.01	61.73 ± 1.25	0.88 ± 0.01	0.54 ± 0.02	N.D.	7.11 ± 0.14	92.89 ± 0.14
PP05_01 (pGG128; empty plasmid)	0.43 ± 0.05	N.D.	N.D.	0.43 ± 0.05	N.D.	N.D.	N.D.
PP05_01 (pSS126; R.r. phaCAB)	0.67 ± 0.01	23.11 ± 1.04	0.15 ± 0.00	0.51 ± 0.02	99.57 ± 0.07	<0.5	<0.5
PP05_01 (pRK182; R.r. phaCABP)	0.60 ± 0.03	25.37 ± 1.59	0.15 ± 0.02	0.45 ± 0.01	100.00	TR.	TR.
PP05_01 (pRK216; C.n. phaCAB)	0.87 ± 0.04	47.13 ± 2.83	0.41 ± 0.05	0.46 ± 0.00	99.87 ± 0.02	<0.5	<0.5
PP05_01 (pMM85; C.n. phaCABP)	1.32 ± 0.10	84.31 ± 0.12	1.11 ± 0.08	0.21 ± 0.02	98.45 ± 0.54	0.98 ± 0.32	0.56 ± 0.21
PP05_01 (pMM106; P.p. phaCABP)	0.68 ± 0.02	44.90 ± 0.08	0.30 ± 0.01	0.37 ± 0.01	100.00	TR.	TR.