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. 2023 Oct 2;42(22):e114334. doi: 10.15252/embj.2023114334

Figure 2. Replisome stalling is dependent on probability of structure‐formation and is affected by orientation.

Figure 2

  1. Replication of G4 substrates containing poly(dG)60 or an increasing number of consecutive (c‐MYC Pu22)n or (GGGT)n repeats. Products were analysed on a denaturing agarose gel.
  2. Replication of iM substrates containing poly(dC)60 or an increasing number of consecutive (DUX4L22)n or (SNORD112)n repeats. Products were analysed on a denaturing agarose gel.
  3. Replication of substrates containing a tract of poly(dG)60, either uninterrupted (lane 2), interrupted every 5th guanine with either a thymine or adenine, or interrupted every 10th guanine with either a thymine or adenine. Replication products were analysed on a denaturing agarose gel.
  4. Replication of substrates containing a tract of poly(dC)60, either uninterrupted, interrupted every 5th cytosine with either an adenine or thymine, or interrupted every 10th cytosine with either an adenine or thymine. Replication products were analysed on a denaturing agarose gel.
  5. Replication products from substrates containing a tract of poly(dG)60, or arrays of either (c‐MYC Pu22)8 or (GGGT)28 on the leading strand template in the forward (F) or the reverse orientation (R). In the forward orientation, the G‐rich strand serve as a template for leading strand synthesis, where in the reverse orientation, the C‐rich strand serves as a leading strand template.
  6. Replication products from substrates containing a tract of poly(dC)60, or arrays of (DAP)4, (DUX4L22)4, or (SNORD112)4 on the leading strand template in the forward (F) or the reverse orientation (R).

Source data are available online for this figure.