Table 1.
Sequences of G4 oligonucleotides and their biophysical properties.
| Substrate | Sequence (5′ ‐> 3′) | Buffer | UV Melting TM (°C) | G4 forming? |
|---|---|---|---|---|
| c‐MYC PU22 | TGAGGGTGGGTAGGGTGGGTAA | KCl | 89.3 | Yes |
| c‐MYC PU22 | TGAGGGTGGGTAGGGTGGGTAA | LiCl | 40.6 | Yes |
| c‐MYC PU22 MUT | TGAGAGTGAGTAGAGTGAGTAA | KCl | 43.2 | No |
| c‐MYC PU22 MUT | TGAGAGTGAGTAGAGTGAGTAA | LiCl | 51.2 | No |
| (GGGT)4 | GGGTGGGTGGGTGGGT | KCl | 91.4 | Yes |
| (GGGT)4 | GGGTGGGTGGGTGGGT | LiCl | 68.3 | Yes |
| (GGGT)3 | GGGTGGGTGGGT | KCl | 61.5 | Yes |
| (GGGT)3 | GGGTGGGTGGGT | LiCl | 40.8 | Not by TDS |
| CEB25L111(T) | AAGGGTGGGTGGGTGGGTGTGAGTGTGGGTGTGGAGGTAGATGT | KCl | 91 | Yes |
| CEB25L111(T) | AAGGGTGGGTGGGTGGGTGTGAGTGTGGGTGTGGAGGTAGATGT | LiCl | 58 | Yes |
| CEB25L111(A) | AAGGGAGGGAGGGAGGGTGTGAGTGTGGGTGTGGAGGTAGATGT | KCl | 83.6 | Yes |
| CEB25L111(A) | AAGGGAGGGAGGGAGGGTGTGAGTGTGGGTGTGGAGGTAGATGT | LiCl | n/a | Not by TDS |
| (TTAGGG)4 | TTAGGGTTAGGGTTAGGGTTAGGG | KCl | 66.2 | Yes |
| (TTAGGG)4 | TTAGGGTTAGGGTTAGGGTTAGGG | LiCl | 41 | Yes |
| Bu1A G4 | GGGCTGGGTGGGTGCTGTCAAGGGCTGGG | KCl | 82.5 | Yes |
| Bu1A G4 | GGGCTGGGTGGGTGCTGTCAAGGGCTGGG | LiCl | 39.7 | Yes |
| (GGGGCC)6 | GGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCC | KCl | n/a | n/a |
| (GGGGCC)6 | GGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCC | LiCl | n/a | n/a |
| Poly(dG)16 | GGGGGGGGGGGGGGGG | KCl | n/a | n/a |
| Poly(dG)16 | GGGGGGGGGGGGGGGG | LiCl | n/a | n/a |