Table 3.
Heating protocol for Uv–Vis melting curve of DNA sequences.
| Stage | Data interval (°C) | Rate (°C/min) | Temperature start/end (°C) |
|---|---|---|---|
| 1 | 1 | 5 | 25/95 |
| 2 | 1 | 5 | 95/25 |
| 3 | 1 | 5 | 25/95 |
| 4 | 1 | 5 | 95/25 |
| 5 | 0.1 | 0.5 | 25/95 |
| 6 | 0.1 | 0.5 | 95/25 |
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