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. 2023 Nov 15;2023(11):CD014911. doi: 10.1002/14651858.CD014911.pub2

Lopes 2018.

Study characteristics
Patient Sampling Retrospective, multicentre, case‐control study. A total of 3693 people were enroled from 5 different clinics. Participants were divided into 2 data sets: 1 training set and 1 validation set. The training set included the preoperative data of the following 3 groups.
  • Stable LASIK cases

  • Ectasia susceptibility

  • Clinical keratoconus


The algorithm was independently tested in a different set of stable LASIK cases and people with very asymmetric ectasia; these people had clinically diagnosed ectasia in 1 eye and normal topography in the fellow eye.
Patient characteristics and setting The participants were grouped as follows.
  • LASIK cases (2980 participants with minimum follow‐up of 7 years)

  • Ectasia susceptibility (71 eyes of 45 participants who developed post‐LASIK ectasia)

  • Clinical keratoconus (182 participants)

  • Participants with very asymmetric ectasia (188 participants); these people had clinically diagnosed ectasia in 1 eye and normal topography in the fellow eye.

Index tests Random forest: multiple decision trees were built and merged to improve accuracy of the prediction. 2 steps of validation were used to assess the generalization and clinical validity of the models and their ability to correctly classify new data. The first was a holdout validation: the training set was randomly split into 2 data sets: the first comprised 70% of the total data set and was used to actually train the models; the other 30% was used to test the model accuracy. The second validation step was an independent test with cases that were not part of the training set. The algorithm analysed the raw tomographic data to identify the different patterns and detect keratoconus.
Target condition and reference standard(s) All eyes were examined by rotating Scheimpflug corneal and anterior segment tomography (Pentacam HR; Oculus GmbH, Wetzlar, Germany). Image quality was checked, so that only cases with acceptable‐quality images were included in the study. 1 experienced fellowship‐trained corneal specialist reviewed all the cases so that they were correctly classified in the keratoconus and very asymmetric ectasia groups. All cases were diagnosed before the algorithm analysed the images.
Flow and timing All eyes received the reference standard and were included in the 2 × 2 table of the index test.
Comparative 5 models were developed and compared: regularized discriminant analysis (RDA), support vector machine (SVM), naïve Bayes (NB), neural networks (NN), and random forest (RF). It is unclear if all tests were developed and interpreted without knowledge of each other and if all data were used for each test.
Notes No funding or grant support.
Methodological quality
Item Authors' judgement Risk of bias Applicability concerns
DOMAIN 1: Patient selection
Was a consecutive or random sample of patients enrolled? No    
Was a case‐control design avoided? No    
Did the study avoid inappropriate exclusions? Unclear    
Could the selection of patients have introduced bias?   High risk  
Are there concerns that the included patients and setting do not match the review question?     High
DOMAIN 2: Index test (All tests)
Were the index test results interpreted without knowledge of the results of the reference standard? Yes    
If a threshold was used, was it pre‐specified? Unclear    
Was the model designed in an appropriate manner? Yes    
Could the conduct or interpretation of the index test have introduced bias?   Low risk  
Are there concerns that the index test, its conduct, or interpretation differ from the review question?     Low concern
DOMAIN 3: Reference standard
Is the reference standard likely to correctly classify the target condition? No    
Were the reference standard results interpreted without knowledge of the results of the index tests? Yes    
Could the reference standard, its conduct, or its interpretation have introduced bias?   High risk  
Are there concerns that the target condition as defined by the reference standard does not match the question?     Low concern
DOMAIN 4: Flow and timing
Did all patients receive the same reference standard? Unclear    
Were all patients included in the analysis? Yes    
Could the patient flow have introduced bias?   Unclear risk  
DOMAIN 5: Comparative
Were different AI tests were developed and interpreted without knowledge of each other. Unclear    
Are the proportions and reasons for missing data similar for all index tests? Unclear    
    Unclear risk