Fig. 7.

Characterization of a deletion mutant lacking Afu4g11540 that encodes a putative glycerol kinase. The deletion mutant and the parental strain AfS35 were compared in a drop dilution assay on AMM (A), AMM with glycerol (1 g/l) as sole carbon source (B) and AMM supplemented with 1 µg/ml fludioxonil (C). Colonies that grew on the glycerol-containing plates were difficult to visualize due to their thin and spread appearance (B); the edges of these colonies are therefore indicated by discontinuous circles. The number of spores per spot is indicated. D shows the glycerol concentrations of cellular extracts obtained either from hyphae after 6 h in the presence of fludioxonil (1 µg/ml) or from non-treated controls