Figure 1.

P. megaterium ZS-3 is a moderately salt-tolerant strain. (a) ZS-3 was inoculated on LB plates supplemented with 0%, 3%, 5%, 7%, or 9% NaCl. Images of strain ZS-3 colony formation were recorded at 36 h. (b) Growth curves for P. megaterium ZS-3 generated from Bioscreen C were monitored for 60 h at 28 °C. OD₆₀₀ measurements were collected automatically every 2 h. Values represent the means of three biological experiments, each in ten technical replicates. (c) The cell dry weight of ZS-3 at different salt concentrations was quantified at 72 h. Values represent the means of three biological experiments, each in three technical replicates. Error bars represent SE. Different lowercase letters above the bars represent significant differences based on one-way ANOVA (p < 0.05).