Figure 3.

ACQA regulates ACC/CPT-1 pathway through the phosphorylation of AMPK. (A) The protein levels of ACC (including its phosphorylated levels) and CPT-1 were assessed via Western blot in HepG2. The relative levels of ACC (B) and CPT-1 (C) were analyzed. (D) The protein levels of AMPK, CaMKK₂ and their phosphorylated levels were assessed through Western blot in HepG2. The relative phosphorylated levels of AMPK (E) and CaMKK₂ (F) were analyzed. (G) Fluorescence intensity of calcium ion probe in HepG2 cells was detected with cell flow cytometer. The total detected cells of each group were 10,000. (H) Relative fluorescence intensity level was analyzed by ratio to the CON group and compared with the OA group. (I) The concentration of cAMP was detected and analyzed using enzyme-labeled apparatus. Data are expressed as mean ± SD, * p < 0.05, ** p < 0.01, *** p < 0.001 and **** p < 0.0001, and compared with OA group, n = 3.