Figure 2.

Meristem regeneration occurs at a predictable distance from the site of laser ablation. (a) Schematic of RMD measurements from the site of laser ablation to the origin of the regenerated meristem. Yellow arrowhead marks the laser ablation site and cyan arrowhead marks the position of the regenerated meristem. Black arrows span between the two locations, denoting the distance from damage. (b) Frequency histogram of RMD measurements collected from ablated gametophytes grown on basal media (n = 85). (c) Heatmap of regenerated meristem frequency in 50 μm intervals, illustrated on a representative gametophyte with concentric rings from 0–400 μm, centered on the site of ablation (marked with a black arrow). Scale bar = 0.25 mm.