Skip to main content
. 2023 Oct 31;24(21):15832. doi: 10.3390/ijms242115832

Figure 6.

Figure 6

NPA, NAA, and IAA dosage during recovery from laser ablation causes abnormalities in marginal meristem regeneration. (a–j,n–a’) Live gametophytes on treatment media 2- or 3-days post-ablation and the same individuals 96 h later. (a–m) Effects of NPA dosage during meristem regeneration. (n–d’) Effects of NAA and IAA dosage during meristem regeneration. (k,b’) Violin plot of regenerated meristem ratio (RMR) values for (k) NPA dosage (n ≥ 22) and (b’) NAA or IAA dosage (n ≥ 9) (dashed line = median; dotted lines = upper and lower quartiles; Kruskal–Wallis test with Dunn’s multiple comparisons test; ns, not significant; *, p ≤ 0.05). (l,c’) Percentage of regenerated individuals from 2- to 7-days post-ablation during treatment with (l) NPA or (c’) NAA and IAA. (m,d’) Percentage of individuals with a regenerated meristem in the basal (solid), apical (dotted), or both (striped) portions of the prothallus (n ≥ 9, Fisher’s exact test; *, p < 0.05; ****, p < 0.0001). Yellow and blue arrowhead pointing at ablated and regenerated meristem, respectively. Scale bar = 0.25 mm.